Construction of a shuttle expression vector with a promoter functioning in both halophilicArchaeaandBacteria
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چکیده
منابع مشابه
construction of vector fields with positive lyapunov exponents
in this thesis our aim is to construct vector field in r3 for which the corresponding one-dimensional maps have certain discontinuities. two kinds of vector fields are considered, the first the lorenz vector field, and the second originally introced here. the latter have chaotic behavior and motivate a class of one-parameter families of maps which have positive lyapunov exponents for an open in...
15 صفحه اولConstruction of a Mammalian IRES-based Expression Vector to Amplify a Bispecific Antibody; Blinatumomab
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Blinatumomab, the bispecific T cell engager, has been demonstrated as the most successful BsAb to date. Throughout the past decade, vector design has great importance for the expression of monoclonal antibody in Chinese hamster ovary (CHO) cells. It has been indicated that expression plasmids based on the elongation factor-1 alpha (EF-1 alpha) gene and DHFR selection marker can be highly effect...
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Construction of a Shuttle Vector for Heterologous Gene Expression in Escherichia coli and Microalgae Anabaena
The construction of an integrative shuttle expression vector and potential utility was reported in Escherichia coli and Anabaena (Nostoc) sp. strain PCC 7120. The vector comprised of the following elements: (a) an intergenic non-coding region from Anabaena to facilitate its genomic integration (b) a strong functional PpsbAI promoter from Anabaena for desired gene expression and (c) neomycin pho...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2013
ISSN: 0378-1097
DOI: 10.1111/1574-6968.12278